(tm) is a rapid, highly sensitive method to detect the presence
of alcohol in saliva and provide an approximation of relative
blood alcohol concentration. Since it was well established
that the concentration of alcohol in saliva is very close
to that in the blood, saliva is the preferred specimen for
alcohol testing, allowing for greater detection sensitivity
than breath testing methods. Alco-Screen (tm) is a very simple,
one-step, 2-minute test requiring no instrumentation, calibration
of special traning to be used effectively, and is convenient
enough to be used any time or any place.
Excessive or inappropriate consumption of alcohol is the most
common and pervasive social problems in our society. It is a
contributory factor to many accidents, injuries and medical
conditions. Screening of individuals for alcohol consumption
is an important method for the identification of individuals who
might be at risk due to alcohol intoxication. Screening also
provides additional benefits as a deterrent against inappropriate
The blood alcohol concentration at which a person becomes
impaired is variable dependent upon the individual. Individual
specific parameters such as physical size, weight, activity level,
eating habits and alcohol tolerance all affect the level of
impairment of the individual.
1. Abstain from placing anything in the mouth for 15 minutes prior to beginning
the test. This includes non-alcoholic drinks, tobacco products, coffee,
breath mints, food, etc.
2. Open the foil package and remove the strip. Observe the reactive pad on the
end of the test strip. The pad should be a light cream color. A strip with
a reagent pad which is dark tan in color or otherwise discolored must be
3. Saturate the reactive pad with saliva from mouth or sputum cup.
Immediately start timer.
4. At 2 miutes observe the color change (if any) in the reactive pad. A color
change of green or blue indicates the presence of alcohol and a positive
result. Results obtained after more than 2 minutes and 30 seconds may
5. Estimate the approximate blood alcohol concentration by comparing the
color of the reagent pad with the color chart appearing on the test package.
Alco-Screen (tm) produces a color change in the presence of saliva alcohol
ranging from a light green-gray color a 0.02% blood alcohol concentration to
a dark blue-gray color near 0.30% blood alcohol concentration. Color blocks
are provided within this range to allow an approximation of blood alcohol
concentration to be made. Alco-Screen (tm) may produce colors that appear
to be between adjacent color blocks.
Alco-Screen (tm) is very sensitive to the presence of alcohol. A green color
that is lighter than the 0.02% color block should be interpreted as being
positive to the presence of alcohol in saliva but less than 0.02%
A result where the reagent pad shows no color change (remains white or
cream colored) should be interpreted as a negative result (no alcohol present).
A result where the edges of the reagent pad produces a slight color but the
majority of the pad remains colorless should be repeated to ensure complete
saturation of the reagent pad with saliva. If the second result is the same,
the results should be interpreted as being negative (no alcohol present).
The integrity of Alco-Screen (tm) may be qualitatively verified using a test
solution prepared by adding 4 drops of 80 proof distilled spirits to 8oz. of
water. This solution should provide a color reaction equal to or higher
(darker) than the 0.04% color block.
The color reaction with alcohol in saliva is somewhat slower and less
intense than with alcohol in aqueous solutions.